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QDD version3.1.2 July 2014
Emese Meglecz, Aix-Marseille University, Marseille, France
emese.meglecz@imbe.fr
http://net.imbe.fr/~emeglecz/qdd.html
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SCRIPT pipe3.pl
INPUT file : /home/qdd/galaxy-dist/database/files/000/dataset_265.dat

OUTPUT files:
String for naming output files: NA
	/home/qdd/galaxy-dist/database/files/000/dataset_269.dat: Information on primers, target region parameters, primer design etc.
	/home/qdd/galaxy-dist/database/files/000/dataset_268.dat: Sequences, where primer design was successful

GENERAL PARAMETERS
System (win/linux): linux
Output folder: /home/qdd/galaxy-dist/tools/qdd/qdd_output/
Path to QDD executables: /home/qdd/galaxy-dist/tools/qdd/
Path to Primer3 executables: /usr/bin/
Primer3 version [1/2]: 2
Delete intremediate files: 1
Sequences are extracted from contigs: 1

PRIMER DESIGN PARAMETERS:
Minimum PCR product size: 90
Maximum PCR product size: 320
PCR size step: 50
PRIMER_GC_CLAMP: 0
PRIMER_OPT_SIZE: 20
PRIMER_MIN_SIZE: 18
PRIMER_MAX_SIZE: 27
PRIMER_OPT_TM: 60
PRIMER_MIN_TM: 57
PRIMER_MAX_TM: 63
PRIMER_MAX_DIFF_TM: 10
PRIMER_MIN_GC: 20.0
PRIMER_OPT_GC_PERCENT: 50.0
PRIMER_MAX_GC: 80.0
PRIMER_SELF_ANY: 8.0
PRIMER_SELF_END: 3.0
PRIMER_MAX_POLY_X: 3
PRIMER_NUM_RETURN: 3
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Total number of input sequences: 5764
Total number of sequences with primers: 5612
Total number of sequences with target MS: 5764

pipe3.pl started at : Mon Jul 21 18:45:19 2025
The analyses took 1128 seconds
The analysis is finished