/isilon_temp/garber/bin/RSEM/rsem-extract-reference-transcripts /isilon_temp/garber/genome_data/c_elegans/ce10/rsem_ref 0 /isilon_temp/garber/genome_data/c_elegans/ce10/ucsc.gtf 1 /isilon_temp/garber/genome_data/c_elegans/ce10/ucsc_into_genesymbol.rsem /isilon_temp/garber/genome_data/c_elegans/ce10/ce10.fa Parsed 200000 lines Parsed 400000 lines Parsing gtf File is done! /isilon_temp/garber/genome_data/c_elegans/ce10/ce10.fa is processed! Extracting sequences is done! Group File is generated! Transcript Information File is generated! Chromosome List File is generated! Extracted Sequences File is generated! /isilon_temp/garber/bin/RSEM/rsem-preref /isilon_temp/garber/genome_data/c_elegans/ce10/rsem_ref.transcripts.fa 0 /isilon_temp/garber/genome_data/c_elegans/ce10/rsem_ref -l 125 Refs.makeRefs finished! Refs.saveRefs finished! /isilon_temp/garber/genome_data/c_elegans/ce10/rsem_ref.transcripts.fa is generated! /isilon_temp/garber/genome_data/c_elegans/ce10/rsem_ref.idx.fa is generated! /isilon_temp/garber/bin/bowtie-0.12.9/bowtie-build -f /isilon_temp/garber/genome_data/c_elegans/ce10/rsem_ref.idx.fa /isilon_temp/garber/genome_data/c_elegans/ce10/rsem_ref Settings: Output files: "/isilon_temp/garber/genome_data/c_elegans/ce10/rsem_ref.*.ebwt" Line rate: 6 (line is 64 bytes) Lines per side: 1 (side is 64 bytes) Offset rate: 5 (one in 32) FTable chars: 10 Strings: unpacked Max bucket size: default Max bucket size, sqrt multiplier: default Max bucket size, len divisor: 4 Difference-cover sample period: 1024 Endianness: little Actual local endianness: little Sanity checking: disabled Assertions: disabled Random seed: 0 Sizeofs: void*:8, int:4, long:8, size_t:8 Input files DNA, FASTA: /isilon_temp/garber/genome_data/c_elegans/ce10/rsem_ref.idx.fa Reading reference sizes Time reading reference sizes: 00:00:02 Calculating joined length Writing header Reserving space for joined string Joining reference sequences Time to join reference sequences: 00:00:00 bmax according to bmaxDivN setting: 10414133 Using parameters --bmax 7810600 --dcv 1024 Doing ahead-of-time memory usage test Passed! Constructing with these parameters: --bmax 7810600 --dcv 1024 Constructing suffix-array element generator Building DifferenceCoverSample Building sPrime Building sPrimeOrder V-Sorting samples V-Sorting samples time: 00:00:02 Allocating rank array Ranking v-sort output Ranking v-sort output time: 00:00:01 Invoking Larsson-Sadakane on ranks Invoking Larsson-Sadakane on ranks time: 00:00:00 Sanity-checking and returning Building samples Reserving space for 12 sample suffixes Generating random suffixes QSorting 12 sample offsets, eliminating duplicates QSorting sample offsets, eliminating duplicates time: 00:00:00 Multikey QSorting 12 samples (Using difference cover) Multikey QSorting samples time: 00:00:00 Calculating bucket sizes Binary sorting into buckets 10% 20% 30% 40% 50% 60% 70% 80% 90% 100% Binary sorting into buckets time: 00:00:05 Splitting and merging Splitting and merging time: 00:00:00 Split 1, merged 7; iterating... Binary sorting into buckets 10% 20% 30% 40% 50% 60% 70% 80% 90% 100% Binary sorting into buckets time: 00:00:03 Splitting and merging Splitting and merging time: 00:00:00 Avg bucket size: 5.95093e+06 (target: 7810599) Converting suffix-array elements to index image Allocating ftab, absorbFtab Entering Ebwt loop Getting block 1 of 7 Reserving size (7810600) for bucket Calculating Z arrays Calculating Z arrays time: 00:00:00 Entering block accumulator loop: 10% 20% 30% 40% 50% 60% 70% 80% 90% 100% Block accumulator loop time: 00:00:00 Sorting block of length 6818495 (Using difference cover) Sorting block time: 00:00:11 Returning block of 6818496 Getting block 2 of 7 Reserving size (7810600) for bucket Calculating Z arrays Calculating Z arrays time: 00:00:00 Entering block accumulator loop: 10% 20% 30% 40% 50% 60% 70% 80% 90% 100% Block accumulator loop time: 00:00:01 Sorting block of length 4742291 (Using difference cover) Sorting block time: 00:00:12 Returning block of 4742292 Getting block 3 of 7 Reserving size (7810600) for bucket Calculating Z arrays Calculating Z arrays time: 00:00:00 Entering block accumulator loop: 10% 20% 30% 40% 50% 60% 70% 80% 90% 100% Block accumulator loop time: 00:00:01 Sorting block of length 7303397 (Using difference cover) Sorting block time: 00:00:20 Returning block of 7303398 Getting block 4 of 7 Reserving size (7810600) for bucket Calculating Z arrays Calculating Z arrays time: 00:00:00 Entering block accumulator loop: 10% 20% 30% 40% 50% 60% 70% 80% 90% 100% Block accumulator loop time: 00:00:01 Sorting block of length 7632051 (Using difference cover) Sorting block time: 00:00:21 Returning block of 7632052 Getting block 5 of 7 Reserving size (7810600) for bucket Calculating Z arrays Calculating Z arrays time: 00:00:00 Entering block accumulator loop: 10% 20% 30% 40% 50% 60% 70% 80% 90% 100% Block accumulator loop time: 00:00:01 Sorting block of length 6347982 (Using difference cover) "/isilon_temp/garber/bin/bowtie-0.12.9/bowtie-build -f /isilon_temp/garber/genome_data/c_elegans/ce10/rsem_ref.idx.fa /isilon_temp/garber/genome_data/c_elegans/ce10/rsem_ref" failed! Plase check if you provide correct parameters/options for the pipeline!