Usage: makeUCSCfile [options] Creates a bedgraph file for visualization using the UCSC Genome Browser General Options: -fsize <#> (Size of file, when gzipped, default: 5e7) -strand (control if reads are separated by strand, default: both) -fragLength <# | auto | given> (Approximate fragment length, default: auto) -adjust <#> (Adjust edge of tag 3' by # bp, negative for 5', default: none[good for dnase]) -tbp <#> (Maximum tags per bp to count, default: no limit) -mintbp <#> (Minimum tags per bp to count, default: no limit) -res <#> (Resolution, in bp, of file, default: 1) -avg (report average coverage if resolution is larger than 1bp, default: max is reported) -lastTag (To keep ucsc happy, last mapped tag is NOT extended by default Using this option will allow extending of data past the last tag position) -norm <#> (Total number of tags to normalize experiment to, default: 1e7) -normLength <#> (Expected length of fragment to normalize to [0=off], default: 100) -noadj (Do not normalize tag counts) -neg (plot negative values, i.e. for - strand transcription) -CpG (Show unmethylated CpG ratios) -color <(0-255),(0-255),(0-255)> (no spaces, rgb color for UCSC track, default: random) -i (normalize bedGraph to input data) -pseudo <#> (Number of pseudo counts used to smooth out low coverage areas, default: 5) -log (report log2 ratio instead of linear ratio) -inputtbp <#>, -inputFragLength <#>, -inputAdjust <#> can also be set -bigWig (creates a full resolution bigWig file and track line file) This requires bedGraphToBigWig to be available in your executable path Also, because how how bigWig files work, use "-strand -" and "-strand +" in separate runs to make strand specific files: "-strand separate" will not work Consider using makeBigWig.pl and makeMultiWigHub.pl if interested in bigWigs -o (send output to this file - will be gzipped, default: prints to stdout) auto: this will place an appropriately named file in the tag directory -name <...> (Name of UCSC track, default: auto generated) -style