cellranger 3.0.2 is located under /share/pkg/cellranger/3.0.2 /share/pkg/cellranger/3.0.2/cellranger-cs/3.0.2/bin cellranger mkref (3.0.2) Copyright (c) 2019 10x Genomics, Inc. All rights reserved. ------------------------------------------------------------------------------- Apr 26 16:20:33 ..... Started STAR run Apr 26 16:20:33 ... Starting to generate Genome files Apr 26 16:21:13 ... starting to sort Suffix Array. This may take a long time... Apr 26 16:21:18 ... sorting Suffix Array chunks and saving them to disk... Apr 26 16:39:57 ... loading chunks from disk, packing SA... Apr 26 16:40:34 ... Finished generating suffix array Apr 26 16:40:34 ... Generating Suffix Array index Apr 26 16:42:14 ... Completed Suffix Array index Apr 26 16:42:14 ..... Processing annotations GTF Apr 26 16:42:25 ..... Inserting junctions into the genome indices Apr 26 16:47:17 ... writing Genome to disk ... Apr 26 16:47:22 ... writing Suffix Array to disk ... Apr 26 16:47:41 ... writing SAindex to disk Apr 26 16:47:46 ..... Finished successfully Creating new reference folder at /share/data/umw_biocore/genome_data/cellranger/zebrafish/GRCz11/GRCz11 ...done Writing genome FASTA file into reference folder... ...done Computing hash of genome FASTA file... ...done Indexing genome FASTA file... ...done Writing genes GTF file into reference folder... ...done Computing hash of genes GTF file... ...done Writing genes index file into reference folder (may take over 10 minutes for a 3Gb genome)... ...done Writing genome metadata JSON file into reference folder... ...done Generating STAR genome index (may take over 8 core hours for a 3Gb genome)... ...done. >>> Reference successfully created! <<< You can now specify this reference on the command line: cellranger --transcriptome=/share/data/umw_biocore/genome_data/cellranger/zebrafish/GRCz11/GRCz11 ... ------------------------------------------------------------ Sender: LSF System Subject: Job 3748714: in cluster Done Job was submitted from host by user in cluster at Fri Apr 26 16:15:28 2019. Job was executed on host(s) , in queue , as user in cluster at Fri Apr 26 16:17:16 2019. was used as the home directory. was used as the working directory. Started at Fri Apr 26 16:17:16 2019. Terminated at Fri Apr 26 16:47:47 2019. Results reported at Fri Apr 26 16:47:47 2019. Your job looked like: ------------------------------------------------------------ # LSBATCH: User input module load cellranger/3.0.2 && cd /share/data/umw_biocore/genome_data/cellranger/zebrafish/GRCz11 && cellranger mkref --genome=GRCz11 --fasta=GRCz11plus_ensembl.fa --genes=ensembl_GRCz11.95.filtered.gtf ------------------------------------------------------------ Successfully completed. Resource usage summary: CPU time : 1738.92 sec. Max Memory : 18987 MB Average Memory : 10406.91 MB Total Requested Memory : 20048.00 MB Delta Memory : 1061.00 MB Max Swap : - Max Processes : 6 Max Threads : 15 Run time : 1831 sec. Turnaround time : 1939 sec. The output (if any) is above this job summary.