singularity singularity-2.6.1 is located under /share/pkg/singularity/singularity-2.6.1 WARNING: Non existent bind point (directory) in container: '/lsf' WARNING: Non existent bind point (directory) in container: '/farline' WARNING: Not mounting requested bind point (already mounted in container): /project WARNING: Not mounting requested bind point (already mounted in container): /share ['/usr/bin/cellranger-6.0.1/bin/rna/mkref', '--genome=GRCz11_v4.3.2_cellranger_v6', '--fasta=danRer11.primary.fa', '--genes=V4.3.2.validated.gtf', '--ref-version=1.0.0'] May 25 01:55:00 ..... started STAR run May 25 01:55:00 ... starting to generate Genome files May 25 01:56:59 ... starting to sort Suffix Array. This may take a long time... May 25 01:57:13 ... sorting Suffix Array chunks and saving them to disk... May 25 02:42:20 ... loading chunks from disk, packing SA... May 25 02:43:00 ... finished generating suffix array May 25 02:43:00 ... generating Suffix Array index May 25 02:46:07 ... completed Suffix Array index May 25 02:46:07 ..... processing annotations GTF May 25 02:46:43 ..... inserting junctions into the genome indices May 25 02:59:52 ... writing Genome to disk ... May 25 03:00:03 ... writing Suffix Array to disk ... May 25 03:00:44 ... writing SAindex to disk May 25 03:00:55 ..... finished successfully Creating new reference folder at /share/data/umw_biocore/dnext_data/genome_data_other/cellranger/zebrafish/GRCz11_v4.3.2_cellranger_v6/GRCz11_v4.3.2_cellranger_v6 ...done Writing genome FASTA file into reference folder... ...done Indexing genome FASTA file... ...done Writing genes GTF file into reference folder... ...done Generating STAR genome index (may take over 8 core hours for a 3Gb genome)... ...done. Writing genome metadata JSON file into reference folder... Computing hash of genome FASTA file... ...done Computing hash of genes GTF file... ...done ...done >>> Reference successfully created! <<< You can now specify this reference on the command line: cellranger --transcriptome=/share/data/umw_biocore/dnext_data/genome_data_other/cellranger/zebrafish/GRCz11_v4.3.2_cellranger_v6/GRCz11_v4.3.2_cellranger_v6 ... ------------------------------------------------------------ Sender: LSF System Subject: Job 9753479: in cluster Done Job was submitted from host by user in cluster at Mon May 24 21:52:17 2021 Job was executed on host(s) , in queue , as user in cluster at Mon May 24 21:52:18 2021 was used as the home directory. was used as the working directory. Started at Mon May 24 21:52:18 2021 Terminated at Mon May 24 23:02:15 2021 Results reported at Mon May 24 23:02:15 2021 Your job looked like: ------------------------------------------------------------ # LSBATCH: User input module load singularity/singularity-2.6.1 && cd /share/data/umw_biocore/genome_data/cellranger/zebrafish/GRCz11_v4.3.2_cellranger_v6 && singularity exec -B /project:/project -B /share:/share /share/data/umw_biocore/dnext_data/singularity/UMMS-Biocore-singularity-cellranger-v4.simg cellranger mkref --genome=GRCz11_v4.3.2_cellranger_v6 --fasta=danRer11.primary.fa --genes=V4.3.2.validated.gtf --ref-version=1.0.0 ------------------------------------------------------------ Successfully completed. Resource usage summary: CPU time : 3991.10 sec. Max Memory : 19022 MB Average Memory : 10747.39 MB Total Requested Memory : 20048.00 MB Delta Memory : 1026.00 MB Max Swap : - Max Processes : 6 Max Threads : 7 Run time : 4204 sec. Turnaround time : 4198 sec. The output (if any) is above this job summary.